Increased Soluble Epoxide Hydrolase in Human Gestational Tissues from Pregnancies Complicated by Acute Chorioamnionitis

Figure 3

Immunoreactivity for sEH in villous tissues from normal pregnant women and women with pregnancies complicated by acute CAM. Immunostaining of CK7 delineates the localization of cytotrophoblasts and syncytiotrophoblasts (a, b). Compared to normal pregnancies, villous tissues from women with acute CAM had increased infiltration of monocytes/macrophages, as demonstrated by positive anti-CD68 immunostaining, within the stroma (d, e). In normal pregnancies, immunostaining of sEH was found mainly in the trophoblast layer and some stromal cells (g). The immunostaining of sEH was generally increased in women with acute CAM compared to normal pregnant women (h). In addition, strong sEH immunostaining was noted in the villous endothelium in women with acute CAM (arrows). There was essentially no staining at all on the negative controls when the primary antibody was substituted with nonimmune IgG (c, f, and i). .