A Peptide Analogue of Selectin Ligands Attenuated Atherosclerosis by Inhibiting Monocyte Activation

Figure 1

IELLQAR inhibits selectin binding to monocytes. PBMCs and THP-1 cells were incubated with increasing concentrations of IELLQAR (1, 5, and 10 μM) in the presence of 10 μg/mL of P-, E-, or L-selectin for 30 min, analyzed by flow cytometry, and plotted as the percent inhibition vs. bovine serum albumin (BSA) control. IELLQAR exhibited dose-dependent inhibitory effects against selectin family binding to monocytes, with the highest inhibition of P-selectin binding to PBMCs and THP-1 cells (a), a moderate inhibition of E-selectin (b), and the lowest inhibition of L-selectin (c). Data are presented as the ().

(a)

(b)

(c)