A Peptide Analogue of Selectin Ligands Attenuated Atherosclerosis by Inhibiting Monocyte Activation

Figure 6

IELLQAR inhibited monocyte activation by P-selectin-dependent activation of the NF-κB pathways. (a) PBMCs were treated with P-selectin (10 μg/mL) for different times. Representative Western blots showing the expression levels of total and phosphorylated IκBα and P65. (b) PBMCs were treated with IELLQAR (1, 3, or 10 μM) and coincubated with or without 10 μg/mL of P-selectin for 30 min. Representative Western blots showing the expression levels of total and phosphorylated IκBα and P65. (c) PBMCs were treated with PBS, LPS (1 μg/ml), P-selectin, IELLQAR (10 μM) plus P-selectin, or IELLQAR plus LPS for 2 h, and the mRNA expression levels of IL-6, TNF-α, and MCP-1 were measured by qRT-PCR. Data are presented as the (). , , and .

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