Research Article
Epigenetic Regulation of IL-17-Induced Chemokines in Lung Epithelial Cells
Figure 7
Epigenetic regulation of IL-17 pathway in mouse airway epithelial cells. Murine lung epithelial (MLE12) cells were cultured with HITES (hydrocortisone, insulin, transferrin, estradiol, and selenium) medium containing 10% fetal bovine serum (FBS) and antibiotics at 37°C in an incubator containing 5% CO2. Cells were treated with control medium, 50 ng/ml m-IL-17A protein, 1 μM 5-azacytidine, 200 nM CPI, 50 ng/ml h-IL-17A plus 1 μM 5-azacytidine and 50 ng/ml h-IL-17A plus 200 nM CPI, respectively. 6 h qPCR was performed for Cxcl1 mRNA production with (a) IL-17A/5-azacytidine and (c) IL-17A/CPI-treated cells. 24 h supernatant was collected to reach CXCL1 protein level by ELISA for (b) IL-17A/5-azacytidine and (d) IL-17A/CPI-treated cells. ; ; ; ; by a one-way ANOVA test. It is the representative figure from 4 experiments.
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