Exploring the Mechanism on the Medullary Visceral Zone Inhibiting the Cholinergic Anti-inflammatory Pathway Induced by Sepsis

Figure 4

Study of the cholinergic anti-inflammatory pathway (CAP) on immune regulation. (a) Representative scatter plot images of flow cytometry of CD4+CD25+Treg and CD4+IL-17+TH17 lymphocyte among different groups. (b) Double positive percentage of CD4+CD25+Treg and CD4+IL-17+TH17 lymphocytes among different groups. (c) The ratio of CD4+CD25+Treg/TH17 among groups. CD4+CD25+Treg lymphocyte percentages of the model group, GTS-21 group, and MLA group were much higher than those of the control group. The value and 95% CI were, respectively, 0.000, -7.893∼-2.754; 0.002, -6.773∼-1.634; and 0.000, -9.846∼-4.707, and so were CD4+IL-17+TH17 lymphocyte percentages; they were 0.000, -2.668∼-1.279; 0.002, -1.851∼-0.462; and 0.000, -3.858∼-2.469. CD4+CD25+Treg and CD4+IL-17+TH17 lymphocyte percentages of the MLA group were much higher than those of the GTS-21 group. The value and 95% CI were, respectively, 0.018, 0.504∼5.643; 0.000, 1.312∼2.701. A: vs. control group; B: vs. model group; C: vs. GTS-21 group.

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