Detection of CD39 and a Highly Glycosylated Isoform of Soluble CD73 in the Plasma of Patients with Cervical Cancer: Correlation with Disease Progression

Figure 3

Catalytic activity of CD39 and CD73 in PFP of NDs and patients with LSILs, HSILs, or CC. Aliquots of 5 μl of PFP from NDs (black bars), patients with LSILs (white bars), patients with HSILs (diagonal lines), and patients with CC (horizontal lines) were incubated in the presence of 5 mM ADP or AMP (total volume 100 μl) and in the presence or absence of POM-1 or APCP, specific inhibitors of CD39 and CD73, respectively. (a) The Ado produced by hydrolysis of the nucleotides was quantified after 72 h by UPLC using standard concentrations of synthetic Ado (upper). A representative linear relationship between the Ado concentration and the optical density is shown (lower). (b) The amount of Ado produced during the incubation of PFP with ADP (upper) or AMP (lower) and in the presence or absence of POM-1 or APCP is shown. Differences in Ado concentrations were analyzed by two-way ANOVA. , . The data represent three independent experiments.

(a)

(b)