Detection of CD39 and a Highly Glycosylated Isoform of Soluble CD73 in the Plasma of Patients with Cervical Cancer: Correlation with Disease Progression

Figure 4

Detection of CD73 in the PFP of NDs and patients with LSILs, HSILs, or CC. Samples of 3 μl of PFP (a) or 20 ng of CD73 contained in the PFP (b) of the ND, LSIL, HSIL, and CC groups were analyzed by Western blot using the anti-CD73 antibody. The densities of the 70 kD (black bars) and 90 kD (white bars) bands detected in the samples of patients with LSILs, HSILs, or CC relative to those of NDs (set to 1) are shown. (c) Twenty-nanogram samples of CD73 contained in PFP were subjected to deglycosylation using the enzymes endoglycosidase H and N-glycanase. The densities of the 70 kD (black bars), 80 kD (bars with horizontal lines), 85 kD (bars with diagonals), and 90 kD (white bars) bands detected in the LSIL, HSIL, and CC samples relative to the ND samples (set to 1) are shown. MW: molecular weight. A representative test of three independent tests is shown.

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