Loss of SNHG4 Attenuated Spinal Nerve Ligation-Triggered Neuropathic Pain through Sponging miR-423-5p

<div>miR-423-5p was a target for SNHG4. (a) Binding regions between miR-423-5p and SNHG4. StarBase was used to predict the target microRNA regulated by SNHG4. (b) The correlation between SNHG4 and Ago2 was evaluated by RIP assay in PC12 cells. Cellular lysates were immunoprecipitated using Ago2 antibody or IgG. SNRNP70 level was employed as a positive control. (c) RNA pull-down assay indicated the direct interaction between miR-423-5p and SNHG4 in PC12 cells. Cellular lysates were pulled down using biotinylated control (NC-Bio), miR-423-5p (miR-423-5p-Bio), or miR-423-5p probe containing mutations in the SNHG4-binding site (miR-423-5p-Bio-mut). Three independent experiments were carried out. 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Mediators of Inflammation

fig5

Figure 5

Figure 5: Loss of SNHG4 Attenuated Spinal Nerve Ligation-Triggered Neuropathic Pain through Sponging miR-423-5p 