Triticum vulgare Extract Modulates Protein-Kinase B and Matrix Metalloproteinases 9 Protein Expression in BV-2 Cells: Bioactivity on Inflammatory Pathway Associated with Molecular Mechanism Wound Healing

Figure 3

MMP9 protein analyses. (a) Heat-map of in silico analyses of protein expression of major proteins involved in inflammatory molecular pathway. The values of fold-charge expression were reported in the right side of the figure. PIA: proinflammatory agent; IAI: anti-inflammatory Agent. (b) Coexpression of p65 and MMP9 in BV-2 cells after treatments. Double immunofluorescence for p65 (green) and MMP9 (red) in BV-2 cells after LPS treatment and control. Upregulation of MMP9 (yellow spectra, cytoplasm) and p65 (Aqua spectra, nuclear). p65 regulation after treatments. Upregulation (by LPS) and downregulation (by TVE) of p65 in the nuclei of BV-2 cells. (c) Quantification of p65 and MMP9 protein expressions. (d) Analyses of MMP9 secretion obtained by ELISA test. ns: not significant differences; ; ; ; .

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