The Depletion of ABI3BP by MicroRNA-183 Promotes the Development of Esophageal Carcinoma

Figure 3

The expression levels of ABI3BP and miR-183 in HEEC, Eca-109, and KYSE-150 cells. Three kinds of cells were cultured and harvested when growing to 80-90%. Total mRNA or protein was extracted and detected by real-time PCR or Western blot analysis. (a) Relative ABI3BP mRNA level was detected by using RT-PCR. Five independent experiments () were repeated for each cell line. (b) Relative miR-183-3p level was detected by using RT-PCR. Five independent experiments () were repeated for each cell line. (c) Relative ABI3BP protein level was detected by Western blot. Five independent experiments () were repeated for each cell line. (d) Quantitative analysis of (c). , one-way ANOVA with post hoc.

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