Leptin Regulated ILC2 Cell through the PI3K/AKT Pathway in Allergic Rhinitis

Figure 3

Proliferation of ILC2 was assessed by using tritiated thymidine incorporation under different stimulation shown in (a). The protein expression of IL-4, IL-5, and IL-13 in the culture supernatant of ILC2 isolated from blood of patients tested by ELISA and mRNA expression of GATA3 and RORα in ILC2 isolated from blood of 10 patients tested by RT-PCR after stimulated by leptin (b–f). The AKT phosphorylation was tested with or without leptin treatment in ILC2s (f). . LY294002, PI3K-specific inhibitor, AG490, JAK inhibitor, SB203580, and MAPK inhibitor. All the stimulators were 100 ng/mL, and stimulation time was 3 days. Cytokine combinations (CC) of IL-25 (10 ng/mL), IL-33 (10 ng/mL), TSLP (10 ng/mL), and IL-2 (50 ng/mL) were added in all the cultures. Three independent tests were performed for every experiment.

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