PDK1/mTOR Signaling in Myeloid Cells Differentially Regulates the Early and Late Stages of Sepsis

Figure 1

Myeloid-specific PDK1/Raptor/Rictor deletion reduced the metabolism of myeloid cells. (a–c) PDK1, Raptor, and Rictor mRNA in myeloid cells and lymphocyte from PDK1, Raptor, and Rictor myeloid-specific knockout mice were quantified by real-time PCR. Data are presented as the . (d–f) PDK1, Raptor, and Rictor protein of the specific knockout mice was detected by western blotting analysis. (g–l) The mean fluorescence index (MFI) of CD71 and CD98 in myeloid cells of indicated mice was analyzed by flow cytometry. Data are representative of three independent experiments.

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