PDK1/mTOR Signaling in Myeloid Cells Differentially Regulates the Early and Late Stages of Sepsis

Figure 3

Myeloid-specific deletion of mTOR core factors Raptor and Rictor aggravated CLP-induced sepsis. (a) Littermate Raptor^fl/fl and Raptor^fl/fl/LyzM-Cre mice were subjected to CLP operation, and the survival time was monitored 5 days after the operation. The comparison of survival in different mice was calculated by the Kaplan-Meier method (). (b–e) The measurement of TNFα, IL-6, IL-1β, and IFN-γ secretion by ELISA from serum of Raptor^fl/fl and Raptor^fl/fl/LyzM-Cre mice at 12 h, 24 h, and 48 h after CLP. The comparison of cytokine levels in different mice was calculated by the Mann–Whitney test. ; . (f) Littermate Rictor^fl/fl and Rictor^fl/fl/LyzM-Cre mice were subjected to CLP operation, and the survival time was monitored 5 days after the operation. The comparison of survival in different mice was calculated by the Kaplan-Meier method (). (g–j) The measurement of TNFα, IL-6, IL-1β, and IFN-γ secretion by ELISA from serum of Rictor^fl/fl and Rictor^fl/fl/LyzM-Cre mice at 12 h, 24 h, and 48 h after CLP. The comparison of cytokine levels in different mice was calculated by the Mann–Whitney test. ; .

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)