Inhibition of GPR91 Reduces Inflammatory Mediators Involved in Active Labor in Myometrium

Figure 2

Effect of GPR91 siRNA knockdown on proinflammatory cytokines. Human primary myometrial cells were transfected with 50 nM siCONT or 50 nM siGPR91 and then treated with 1 ng/ml IL1B or 10 ng/ml TNF ( patients). (a–c, e–h) GM-CSF, IL1A, IL1B, and IL6 mRNA expression was analysed by RT-qPCR. (d, i) The concentration of IL6 in the incubation medium was assayed by ELISA. For all data, the fold change was calculated relative to siCONT+IL1B- or siCONT+TNF-transfected cells. Individual data points represent 6-8 independent experiments, and the horizontal line represents the of each group. , repeated measures one-way ANOVA.

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