Inhibition of GPR91 Reduces Inflammatory Mediators Involved in Active Labor in Myometrium

Figure 4

Effect of GPR91 siRNA knockdown on myometrial cell contractility. (a–f) Human primary myometrial cells were transfected with 50 nM siCONT or 50 nM siGPR91 and then treated with (a–d) 1 ng/ml IL1B or (e–h) 10 ng/ml TNF ( patients). (a, c, d, e, g, and h) PTGS2, PTGFR, and CX43 mRNA expression was analysed by RT-qPCR. (b, f) The concentration of PGF_2α in the incubation medium was assayed by ELISA. Individual data points represent 6 independent experiments, and the horizontal line represents the of each group. , repeated measures one-way ANOVA. (i) Cell contraction assays were performed using collagen gels made from human primary myometrial cells transfected with 50 nM siCONT or 50 nM siGPR91 for 48 h ( patients). The collagen gels were then treated with or without 10 ng/ml TNF for 36 h, and the area of gel was determined. Representative gel contraction image from 1 patient is also shown. For all data, the fold change was calculated relative to siCONT+IL1B- or siCONT+TNF-transfected cells. Individual data points represent 6 independent experiments, and the horizontal line represents the of each group. , repeated measures one-way ANOVA.

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