Inhibition of GPR91 Reduces Inflammatory Mediators Involved in Active Labor in Myometrium

Figure 5

Effect of GPR91 siRNA knockdown on RELA transcriptional activity. Human myometrial cells were transfected with 0.75 ng RELA reporter construct. After 6 h, cells were transfected with 50 nM siCONT or 50 nM siGPR91 for 48 h, then treated with (a) 1 ng/ml IL1B ( patients) or (b) 10 ng/ml TNF for an additional 20 h ( patients). RELA promoter activity (normalised to Renilla) is expressed as a ratio of luciferase activity. For all data, the fold change was calculated relative to siCONT+IL1B- or siCONT+TNF-transfected cells. Individual data points represent independent experiments, and the horizontal line represents the of each group. , repeated measures one-way ANOVA.

(a)

(b)