hnRNPA2/B1 Ameliorates LPS-Induced Endothelial Injury through NF-κB Pathway and VE-Cadherin/β-Catenin Signaling Modulation In Vitro

Figure 5

The effect of hnRNPA2/B1 reverses the LPS-induced downregulation of β-catenin and endothelial hyperpermeability in HUVECs. Twelve hours after LPS treatment, HUVECs, and culture supernatant were collected. (a) The mRNA expression level of β-catenin was measured by RT-qPCR. β-Actin was used as a normalization control. (b, c, e, f) The protein expression level of β-catenin was detected by Western blotting. (d) Immunofluorescent staining for β-catenin (green) in HUVECs stained with DAPI (blue). Arrows indicate areas of cell-cell adhesion. Scale bars, 50 μm. Data are expressed as (), vs. Negative control (siRNA/plasmid) .

(a)

(b)

(c)

(d)

(e)

(f)