Sodium Butyrate Protects N2a Cells against Aβ Toxicity In Vitro

Figure 5

NaB suppresses APP expression and increases the BDNF/NEP level in a GPR109A-dependent manner in the protein level. The N2a cells were pretreated for 1 hour with 1 mM PTX and then incubated for 24 hours with 2 mM NaB. (a) Protein expression of APP, NEP, BDNF, and GPR109A in N2a cells was assessed by western blotting (); β-tubulin was used as an internal loading control. (b–e) Quantitative analysis of the expression of APP, BDNF, NEP, and GPR109A of no treatment (NT), NaB-treated (NaB), and PTX and NaB-treated (PTX+NaB) (, , Student’s -test, , , , and ). 2 mM NaB reduced expression of APP and increased expression of BDNF and NEP. Simultaneously, NaB promoted the expression of the GPR109A.

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