Glycogen Synthase Kinase 3β Promotes Postoperative Cognitive Dysfunction by Inducing the M1 Polarization and Migration of Microglia

Figure 5

LPS-induced microglia activation and migration are suppressed by GSK-3β inhibition in vitro. The primary microglia were pretreated with LiCl (1 mM) for 30 min and then incubated with LPS (10 ng/ml) for 24 h. (a) Activation of primary microglia was examined using immunofluorescence. The microglia cells were stained with Iba1 antibody. Expression of Iba1 expression (green) in activated microglia was visualized by confocal microscopy. The blue staining represents DAPI. . (b) Crystal violet staining of primary microglia that migrated into the lower surface of the polycarbonate membrane inserts (8 μm pore size) at 24 h after seeding. . Graph demonstrates the average number of migrating cells per visual field in six random fields. The data are presented as (). and compared with the control group. ^# compared with the LPS group.

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