Leptin Receptor Deficiency Protects Mice against Chronic Cerebral Hypoperfusion-Induced Neuroinflammation and White Matter Lesions

Figure 6

Expression of inflammatory cytokines in the corpus callosum of wild-type and db/db mice with sham or BCAS operation for four weeks. Relative mRNA expression levels of proinflammatory cytokines TNF-α (a), IL-1β (b), and IL-6 (c), and anti-inflammatory cytokines IL-4 (d), IL1Ra (e), and IL-10 (f) against GAPDH were examined by real-time PCR. M1/M2 microglial phenotypes in the mice corpus callosum were examined by immunofluorescence staining. Anti-CD16/32 antibodies were used to label M1-positive microglia, and anti-CD206 antibodies were used to label M2-positive microglia (g). In (a–d), comparisons between different time points were analyzed using Fisher’s LSD test; in (e), comparison between different time points was analyzed using Dunnett’s test; in (f), comparison between different time points in the wild-type group was analyzed using Kruskal Wallis test, and comparison between different time points in db/db group was analyzed using Fisher’s LSD test. , , , and . Wild-type: wild-type mice, Db/db: db/db mice.

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