The Mast Cell Is an Early Activator of Lipopolysaccharide-Induced Neuroinflammation and Blood-Brain Barrier Dysfunction in the Hippocampus

Figure 6

Glial changes after LPS treatment in the hippocampi of WT and Kit^W-sh/W-sh mice. (a) Immunofluorescence staining was used to detect ionized calcium binding adaptor molecule 1 (IBA1), a marker of microglia. . (b) Quantification of IBA1-positive cells in the CA1 of the hippocampus. (c) Protein levels of IBA1 and glial fibrillary acidic protein (GFAP) in the hippocampus were detected by western blotting. (d) Expression levels of IBA1 and GFAP were quantified and normalized to Tubulin levels. Each value was expressed relative to that of the WT+saline group, which was set to 100. (e) Immunofluorescence staining was used to detect GFAP, a marker of astrocytes. . (f) Quantification of GFAP-positive cells in the CA1 of the hippocampus. and vs. the WT+LPS group. Data are presented as the ().

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