The Mast Cell Is an Early Activator of Lipopolysaccharide-Induced Neuroinflammation and Blood-Brain Barrier Dysfunction in the Hippocampus

Figure 7

LPS-induced blood-brain barrier hyperpermeability is reduced in the hippocampi of Kit^W-sh/W-sh mice. (a) Representative images acquired by confocal microscopy show the albumin levels in the CA1 of the hippocampus. The arrow indicates high albumin immunoreactivity in the CA1. . (b) Quantitative data of mean intensities of albumin fluorescence. (c) Protein levels of albumin were detected by western blotting. Expression levels of albumin were quantified and normalized to β-actin levels. (d) The quantitative analysis of Evan’s blue leakage in the hippocampus. (e) Expression levels of occludin and claudin-5 were detected in the hippocampus by western blotting. (f, g) Expression levels of occludin and claudin-5 were quantified and normalized to β-actin levels. Each value was expressed relative to the value of the WT+saline group, which was set to 100. and vs. the WT+LPS group. Data are presented as the ().

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