miR-199a-5p Exacerbated Intestinal Barrier Dysfunction through Inhibiting Surfactant Protein D and Activating NF-κB Pathway in Sepsis

Figure 6

SP-D was a direct target of miR-199a-5p. (a) The luciferase reporter constructs containing the wild type (WT-SP-D) or mutant SP-D (MUT-SP-D) sequence. (b) WT-SP-D or MUT-SP-D was cotransfected into HEK-293T cells with miR-199a-5p mimics or their corresponding negative controls. (c) WT-SP-D or MUT-SP-D was cotransfected into HEK-293T cells with miR-199a-5p inhibitors or their corresponding negative controls. (d) Protein expression of SP-D in intestinal epithelial tissue of the mice. (e) IHC staining of SP-D in the intestinal epithelial tissue of the mice. Three independent experiments were carried out. Error bars stand for the of at least triplicate experiments. .

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