[Retracted] VGLL4 Protects against Oxidized-LDL-Induced Endothelial Cell Dysfunction and Inflammation by Activating Hippo-YAP/TEAD1 Signaling Pathway

Figure 2

Overexpression of VGLL4 alleviates Ox-LDL-induced oxidative stress and inflammation in HUVECs. The HUVECs were treated with Ox-LDL (100 μg/mL) and transfected with the control vector (pcDNA3.1 group) or pcDNA3.1-VGLL4 (p-VGLL4 group), respectively. (a) The protein expression of VGLL4 in the untreated, control, pcDNA3.1, and p-VGLL4 groups. (b) The ROS level in the untreated, control, pcDNA3.1, and p-VGLL4 groups. (c) The NAD⁺ level in the untreated, control, pcDNA3.1, and p-VGLL4 groups. (d) The ATP level in the untreated, control, pcDNA3.1, and p-VGLL4 groups. (e) The IL-6 level in the untreated, control, pcDNA3.1, and p-VGLL4 groups. (f) The TNF-α level in the untreated, control, pcDNA3.1, and p-VGLL4 groups. “” means compared with the untreated group at , and “#” means compared with the pcDNA3.1 group at . GAPDH was used as an invariant internal control for calculating protein fold changes.

(a)

(b)

(c)

(d)

(e)

(f)