Cyr61 Promotes Inflammation of a Gouty Arthritis Model in Rats

Figure 2

(a) The pictures of arthritis from the MSU-induced murine gout model group and the shRNA group after 72 h. (b) The ratio of the right foot to the left foot (normal control) from 0 h to 72 h (, vs. blank). (c) The protein level of Cyr61 was verified by western blot in the rat synovial tissue from the blank or MSU-induced gouty model groups with or without shRNA interference and colchicine as the positive control. (d) Protein level in different groups was expressed as a ratio to β-actin (, vs. blank; ^#, vs. MSU). (e) Relative expression of Cyr61, IL-1β, IL-1, and TNF-α mRNA in the same groups (, vs. blank; ^#, vs. MSU). (f) The concentrations of Cyr61, IL-1β, IL-1, and TNF-α were verified by ELISA in the same groups (, vs. blank; ^#, vs. MSU; ^△, vs. MSU+shRNA).

(a)

(b)

(c)

(d)

(e)

(f)