Research Article
Monocyte Chemotactic Protein 1-Induced Protein 1 Is Highly Expressed in Inflammatory Bowel Disease and Negatively Regulates Neutrophil Activities
Figure 8
Overexpression of MCPIP-1 degrades the mRNA and protein levels of proinflammatory cytokines in neutrophils. Neutrophils () were isolated from the bone marrow of McpipMye-tg () and WT () mice and stimulated with LPS (100 ng/mL) in vitro for 3 hours. One part of the neutrophils () was harvested, and expression of IL-6 (a), IL-1β (b), TNF-α (c), and IFN-γ (d) was analyzed by quantitative RT-PCR and normalized to GAPDH. The rest of the cells () was collected and incubated in the replenished medium for another 24 hours. Supernatants were then harvested, and protein levels of IL-6 (e), IL-1β (f), TNF-α (g), and IFN-γ (h) were measured by ELISA. , , , and .
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