Cangfudaotan Decoction Alleviates Insulin Resistance and Improves Follicular Development in Rats with Polycystic Ovary Syndrome via IGF-1-PI3K/Akt-Bax/Bcl-2 Pathway

Figure 5

CFD ameliorates cell viability, inhibiting cell apoptosis in granular cells with PCOS (). (a) Cell morphology identification of the normal and PCOS model granular cells (magnification ×100); (b) H&E staining of the normal and PCOS model granular cells (magnification ×200); (c) granular cells were treated with CFD (0.1 mg/ml, 0.2 mg/ml) for 12 h, 24 h, 48 h, and 72 h, and then, CCK-8 assay was used to assess cell viability; (d) cell apoptosis was assessed using Annexin V/FITC/PI flow cytometry analysis.