Effects of the Glutamine Administration on T Helper Cell Regulation and Inflammatory Response in Obese Mice Complicated with Polymicrobial Sepsis

Figure 6

Expressions of genes related to macrophage infiltration, tissue injury, and antiapoptosis in the lungs. Cluster of differentiation 68 (CD68), epidermal growth factor-like module-containing mucin-like hormone receptor-like (EMR)-1, zonula occludens (ZO)-1, and antiapoptotic marker B cell lymphoma-extra large (Bcl-xL). SH: sham-operated group; SS: sepsis group with saline; SG: sepsis group with glutamine. mRNA changes were quantitated and analyzed by a real-time PCR and calculated by the comparative CT (2^-ΔΔCt) method. mRNA expression levels in the normal control group were used as a calibrator. Data are shown as the . for each group at 12, 24, or 48 h after cecal ligation and puncture (CLP). Differences among groups at each time point were analyzed by a one-way ANOVA followed by Tukey’s post-hoc test. The symbol “^#” significantly differs from the SH group; the symbol “” significantly differs from the SS group ().