Effects of the Glutamine Administration on T Helper Cell Regulation and Inflammatory Response in Obese Mice Complicated with Polymicrobial Sepsis

Table 2

Sequences of oligonucleotide primers used for PCR amplification.

		Primer sequences (5⟶3)
GAPDH	Forward	AACGACCCCTTCATTGAC
	Reverse	TCCACGACATACTCAGCAC
TNF-α	Forward	ATGGCCTCCCTCTCATCAGT
	Reverse	TTTGCTACGACGTGGGCTAC
IL-1β	Forward	TGCCACCTTTTGACAGTGATG
	Reverse	ATGTGCTGCTGCGAGATTTG
IL-6	Forward	TCCTACCCCAACTTCCAATGCTC
	Reverse	TTGGATGGTCTTGGTCCTTAGCC
CD68	Forward	TGTTCAGCTCCAAGCCCAAA
	Reverse	ACTCGGGCTCTGATGTAGGT
EMR-1	Forward	ACCTTGTGGTCCTAACTCAGTC
	Reverse	ACAAAGCCTGGTTGACAGGTA
ZO-1	Forward	GGCACATCAGCACGATTTCT
	Reverse	CCACAAAAGAAATCCTTTCACACCT
Occludin	Forward	ACTGGGTCAGGGAATATCCA
	Reverse	TCAGCAGCAGCCATGTACTC
Bcl-xL	Forward	ACCTCCTCCCCGACCTATGA
	Reverse	CTATCTCCGGCGACCAGCAA

GAPDH: glyceraldehyde 3-phosphate dehydrogenase; TNF: tumor necrosis factor; IL: interleukin; CD68: cluster of differentiation 68; EMR-1: epidermal growth factor-like module-containing mucin-like hormone receptor-like 1; ZO-1: zonula occludens, Bcl-xL: B cell lymphoma-extra large.