Identification of miRNA-mRNA Crosstalk in Respiratory Syncytial Virus- (RSV-) Associated Pediatric Pneumonia through Integrated miRNAome and Transcriptome Analysis

Figure 4

Validation of differentially expressed miRNA expression levels by qRT-PCR. (a) Relative quantities of 14 miRNAs were analyzed for differential expression between groups. Numbers in each group: mild: 5; severe: 5. (b) Quantification of the significantly upregulated miRNAs in (a). Numbers in each group: mild: 15; severe: 15. Data are shown as from three independent experiments. and (Student’s -test). ns: not significant. (c) The principle component analysis (PCA) of the significantly upregulated miRNAs in (b). Mild: children with mild RSV-associated pneumonia; Severe: children with severe RSV-associated pneumonia.

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