Notch1 Signaling Contributes to Mechanical Allodynia Associated with Cyclophosphamide-Induced Cystitis by Promoting Microglia Activation and Neuroinflammation

Figure 1

Notch1 signaling was upregulated in the spinal dorsal horn (SDH) of the CYP-induced cystitis model. (a) The mechanical withdrawal threshold of the CYP-induced cystitis model was significantly reduced since the first CYP injection and remained for at least 17 days. per group. The data were analyzed by two-way ANOVA followed by the Tukey post hoc test. (b) The expressions of Notch1 protein were significantly upregulated at all three time points (days 8, 12, and 17 after the onset of CYP injection). And the expression of Notch1 was negatively correlated with the mechanical withdrawal threshold of the cystitis animals. per group. (c) The expressions of NICD protein were significantly upregulated at all three time points. And the expression of NICD was also negatively correlated with the mechanical withdrawal threshold of the cystitis animals. per group. Data in (b) and (c) were analyzed by one-way ANOVA followed by the Tukey post hoc test and linear regression. The correlation coefficients were calculated. , , and vs. saline group.

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