Notch1 Signaling Contributes to Mechanical Allodynia Associated with Cyclophosphamide-Induced Cystitis by Promoting Microglia Activation and Neuroinflammation

Figure 2

Inhibition of Notch1 signaling attenuated mechanical allodynia in the CYP-induced cystitis model. (a) Intrathecal injection of DAPT (a γ-secretase and Notch1 signaling inhibitor) the day after every CYP injection could reverse the reduced mechanical withdrawal threshold of cystitis animals to the basal level. In comparison, the mechanical withdrawal threshold of the CYP-induced cystitis animals without DAPT treatment persisted at a low level for at least 12 days. per group. (b) Intrathecal injection of DAPT after the establishment of the CYP-induced cystitis model for three consecutive days could gradually reverse the decrease of mechanical withdrawal threshold in the cystitis animals. In comparison, the threshold of the cystitis animals without DAPT treatment persisted at a low level for at least 15 days. per group. The data in (a) and (b) were analyzed by two-way ANOVA followed by the Tukey post hoc test. (c) The overexpression of Notch1 and NICD in the SDH of cystitis animals in (b) was neutralized by DAPT treatment. per group. Data were analyzed by one-way ANOVA followed by the Tukey post hoc test. vs. CON group. ^### vs. CYP group.

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