Effect of Lipopolysaccharide and TNFα on Neuronal Ascorbic Acid Uptake

Figure 5

NF-κB signaling pathway plays a role in mediating the LPS-induced inhibitory effect on SVCT2 functional expression. SH-SY5Y cells were pretreated with celastrol (100 nM) for 5 h before LPS treatment; then 48 h later, the NF-κB expression in the nucleus (a), IKKαβ expression in the cytoplasm (b), carrier-mediated AA uptake (c), hSVCT2 protein expression (d), hSVCT2 mRNA expression (e), and SLC23A2 promoter activity (f) were determined. @: cells exposed to LPS plus celastrol treatment significantly recovered versus LPS alone-treated cells. Values are of at least 3 separate investigations. ; ; .

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