Effect of Lipopolysaccharide and TNFα on Neuronal Ascorbic Acid Uptake

Table 1

Oligonucleotide primer combinations used to amplify coding region of the respective genes by RT-qPCR.

Gene name	Forward and reverse primers (5-3)
Real-time PCR primers
hSVCT2	TCTTTGTGCTTGGATTTTCGAT; ACGTTCAACACTTGATCGATTC
hRFVT2	CCCTGGTCCAGACCCTA; ACACCCATGGCCAGGA
hSp1	CCATACCCCTTAACCCCG; GAATTTTCACTAATGTTTCCCACC
hβ-actin	CATCCTGCGTCTGGACCT; TAATGTCACGCACGATTTCC
mSVCT2	AACGGCAGAGCTGTTGGA; GAAAATCGTCAGCATGGCAA
mNLRP3	ATTACCCGCCCGAGAAAGG; TCGCAGCAAAGATCCACACAG
mTNFα	CATCTTCTCAAAATTCGAGTGACAA; TGGGAGTAGACAAGGTACAACCC
mβ-actin	ATCCTCTTCCTCCCTGGA; TTCATGGATGCCACAGGA
hnRNA primers
mSVCT2	ACTCTTGTCCATGGCTCTGG; GGGCAAAATCTTCGTTGGGT
mβ-actin	AGATGACCCAGGTCAGTATC; GAGCAGAAACTGCAAAGAT