Prophylactic Treatment of Probiotic and Metformin Mitigates Ethanol-Induced Intestinal Barrier Injury: In Vitro, In Vivo, and In Silico Approaches

Table 1

The list of Homo sapiens primers used for the quantification of mRNA expression levels in qRT-PCR.

Sr. no.	Name of the gene	Forward primer sequence	Reverse primer sequence
1	18S	ACGGAAGGGCACCACCAGGA	CACCACCACCCACGGAATCG
2	ZO-1	TATTATGGCACATCAGCACG	TGGGCAAACAGACCAAGC
3	Occludin	CTCGAGAAAGTGCTGAGTGCCTGGAC	AAGCTTTCGGTGACCAATTCACCTGA
4	CYP2E1	AGGGTACCATGTCTGCCCTCGGAGTGA	ACAATTTGAAAGCTTGTTTGAAAGCGG
5	NOX	GTACAAATTCCAGTGTGCAGACCAC	GTACAAATTCCAGTGTGCAGACCAC
6	TNF-α	CCCTCACACTCAGATCATCTTCT	GCTACGACGTGGGCTACAG
7	IL-6	GACAACTTTGGCATTGTGG	ATGCAGGGATGATGTTCTG
8	IL-10	ACTGCTAACCGACTCCTTA	TAAGGAGTCGGTTAGCAGT