Prophylactic Treatment of Probiotic and Metformin Mitigates Ethanol-Induced Intestinal Barrier Injury: In Vitro, In Vivo, and In Silico Approaches

Table 2

The list of Rattus norvegicus primers used for the quantification of mRNA expression levels in qRT-PCR.

Sr. no.	Name of the gene	Forward primer sequence	Reverse primer sequence
1	18S	GTTGGTTTTCGGAACTGAGGC	GTCGGCATCGTTTATGGTCG
2	ZO-1	TCGGAGCTCGGGCATTATTC	CAGGGCACCATACCAACCAT
3	Occludin	CAACGGCAAAGTGAATGGCA	CTTTCCCCTTCGTGGGAGTC
4	Nrf-2	CAGAGTTTCTTCGCCAGAGG	TGAGTGTGAGGACCCATCG
5	HO-1	CAAATCCCACCTTGAACACA	CGACTGACTAATGGCAGCAG
6	CYP2E1	TCAATCTCT GGACCCCAACTG	GCGCTCTGCACTGTGCTTT
7	NOX	TGACAGTGATGTATGCAGCAT	CAGCTTGTTGTGTGCACGCTG
8	CHOP	ACCACCACACCTGAAAGCA	AGCTGGACACTGTCTCAAAGG
9	Grp78	CCGTAACAATCAAGGTCTACGA	AAGGTGACTTCAATCTGGGGTA
10	TNF-α	TCTCATTCCTGCTCGTGGCG	GGTGAGGAGCACGTAGTCGG
11	IL-6	TTGACAGCCACTGCCTTCCC	CGGAACTCCAGAAGACCAGAGC
12	IL-10	TGCCTTCAGTCAAGTGAAGAC	AAACTCATTCATGGCCTTGTA
13	AMPK	GCTGTGGATCGCCAAATTAT	GCATCAGCAGAGTGGCAATA
14	SREBP-1c	TCTGCCTTGATGAAGTGTGG	AGCAGCCCCTAGAACAAACA
15	FASN	CCTCAGTCCTGTTATCACCCGA	GCTGAATACGACCACGCACTA
16	ACC	CCTTCTTCTACTGGCGACTGAG	TAAGCCTTCACTGTGCCTTCC
17	GPR109A	ACTTTCTGGTGATAAACGGCAAGA	GACTGTCAGGCCGATGGTG
18	SLC5A8	AGCCAGCACTCAGCGTATTT	TTTGAGCTCCAATTCCAACC