Research Article

Chemokine Profiles Are Affected in Serum of Patients with Acute Rejection of Kidney Allograft

Figure 4

Renal proximal tubular cells (RPTECs) were cultured until their confluency and then cocultured with THP-1 cells (monocyte/macrophage cell line separated with 0.4 μm pore filters, stimulated with TNF alpha (10 ng/ml)). After 24 hours, the supernatants were collected and concentrations of chemokines attracting mainly neutrophils (a) CXCL1, (b) CXCL5, (c) CXCL6, and (d) CXCL8 were measured. Data represent one of repetitive experiments done in triplicate and are expressed as ; ; ; .
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