BMSCs Regulate Astrocytes through TSG-6 to Protect the Blood-Brain Barrier after Subarachnoid Hemorrhage

Figure 5

Inhibition of the MAPK signaling pathway to regulate astrocytes. (a) qRT-PCR detected p38 expression. (b) ELISA to measure the contents of IFN-γ, IL-1β, IL-6, and TGF-β1. (c) qRT-PCR examined the expression of iNOS. (d) Western Blot examined the protein expression of iNOS. (e) Immunofluorescence detection of occludin, claudin 3, and ZO-1 fluorescence intensity (scale bar, 100 μm). (f) Flow cytometry was used to measure the apoptosis rate of astrocytes. Compared with the si-NC group, . The above experimental results were all measurement data. Data are expressed as the . The unpaired -test was used between the two groups.

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