Agnuside Alleviates Synovitis and Fibrosis in Knee Osteoarthritis through the Inhibition of HIF-1α and NLRP3 Inflammasome

Figure 4

Agnuside inhibits HIF-1α accumulation and NLRP3 inflammasome activation in LPS-treated FLSs. (a) Relative caspase-1 activity for each group. , in comparison with the normal group. ^#, in comparison with the LPS group. (b) mRNA level of HIF-1α, caspase-1, ASC, and NLRP3 in each group. , , in comparison with the normal group. ^#, ^##, in comparison with the KOA group. (c) Typical protein bands for each group. (d) Protein level comparison of HIF-1α, pro-caspase-1, caspase-1 p10, ASC, and NLRP3 between groups. , compared with the normal group. ^##, compared with the KOA group. (e) FLS supernatant contents of IL-1β and IL-18 between groups. , compared with the normal group. ^##, compared with the KOA group.

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