Agnuside Alleviates Synovitis and Fibrosis in Knee Osteoarthritis through the Inhibition of HIF-1α and NLRP3 Inflammasome

Table 1

Nucleotide sequences of primers used for RT-PCR amplification.

Target gene	Forward primer	Reverse primer
HIF-1α	CCGCAACTGCCACCACTGATG	TGAGGCTGTCCGACTGTGAGTAC
Caspase-1	ATGGCCGACAAGGTCCTGAGG	GTGACATGATCGCACAGGTCTCG
NLRP3	GAGCTGGACCTCAGTGACAATGC	ACCAATGCGAGATCCTGACAACAC
ASC	AGAGTCTGGAGCTGTGGCTACTG	ATGAGTGCTTGCCTGTGTTGGTC
TGF-β	GCAACAATTCCTGGCGTTACCTTG	TGTATTCCGTCTCCTTGGTTCAGC
VEGF	AGCGTTCACTGTGAGCCTTGTTC	CCGCCTTGGCTTGTCACATCTG
TIMP1	GCGTTCTGCAACTCGGACCTG	GTGTAGGCGAACCGGATATCTGTG
GAPDH	GGCCTTCCGTGTTCCTACC	ACTCGACACCTGCCCTCA