Human Acellular Amniotic Matrix with Previously Seeded Umbilical Cord Mesenchymal Stem Cells Restores Endometrial Function in a Rat Model of Injury

Figure 3

UCMSC-AAM transplantation promotes endometrial thickness, mRNA and protein expression, and regulates inflammation factors of injured endometrium. (a) Hematoxylin and eosin (H&E) staining of uterine in the injury group, the AAM group, the UCMSC-AAM group, and the normal group. Scale bars, 500 μm, 25 μm, and 50 μm. The black arrows indicate epithelial cells, and the red arrows indicate glands. Each experiment was repeated four times. Statistical analysis of the endometrial thickness measured by Image-Pro Plus 6.0 software. , , . (b) Immunohistochemical staining of keratin, vimentin, and integrin β3 for epithelial cells, stromal cells, and endometrial receptivity in endometrium of the injury group, the AAM group, the UCMSC-AAM group, and the normal group. Scale bars, 100 μm. Statistical analysis of IOD (Integral optical density) value of positive areas measured by Image-Pro Plus 6.0 software. , , . (c) Relative mRNA expressions of keratin, vimentin, and integrin β3 in endometrium tissues of rats in the injury group, the AAM group, the UCMSC-AAM group, and the normal group that were compared. The relative expression is the average of . , , . (d) Relative mRNA expressions of IL-2, TNFα, IFN-γ, IL-4, IL-10, VEGF, MMP9, and ki67 mRNA in endometrium tissues of rats in the injury group, the AAM group, the UCMSC-AAM group, and the normal group that were compared. The relative expression is the average of . , , . (e) HE staining of heart, liver, spleen, lung, and kidney in the injury group, the AAM group, the UCMSC-AAM group, and the normal group.

(a)

(b)

(c)

(d)

(e)