Research Article
Astaxanthin Inhibits Interleukin-6 Expression in Cerulein/Resistin-Stimulated Pancreatic Acinar Cells
Figure 1
Resistin enhances cerulein-induced reactive oxygen species (ROS) production and NADPH oxidase activation in AR42J cells. (a) The cells were stimulated with cerulein/resistin for the indicated times. (b–d) The cells were stimulated with cerulein with or without resistin for 45 min. (a, b) Intracellular ROS levels were measured by dichlorofluorescein diacetate (DCF-DA) fluorescence. ROS levels were expressed as the relative increase. (c) Representative images of ROS-induced fluorescence response of DCF-DA. (d) NADPH oxidase activity in membrane fractions was measured by the lucigenin assay. Data are expressed as the . of three different experiments. The value for cells without cerulein stimulation in the absence of resistin treatment (none) is set as 100%. vs. 0 min (a) or none (untreated cells; b, c); + vs. cerulein (cells treated with cerulein alone).
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