Astaxanthin Inhibits Interleukin-6 Expression in Cerulein/Resistin-Stimulated Pancreatic Acinar Cells

Figure 2

Resistin enhances cerulein-induced Ca²⁺ overload and NF-κB activation in AR42J cells. (a) The cells were stimulated with cerulein in the presence or absence of resistin for the indicated times. Ca²⁺ level was determined by measuring the fluorescence changes of fluo-4 AM at excitation and emission wavelengths of 494 nm and 525 nm, respectively. Ca²⁺ levels were expressed as , where is the resting background fluorescence, and is fluorescence change over time after treatment with or without cerulein in the presence or absence of resistin. Fluorescence transient changes (obtained within 1-5 min) were plotted (A). (B) Ca²⁺ levels, expressed as , of the cells. Data are expressed as the of three different experiments. Ca²⁺ level in none (untreated cells) was set as 1. vs. 0 min (a) or none (untreated cells; b, c); ⁺ vs. cerulein (cells treated with cerulein alone). (b) The cells were stimulated with cerulein/resistin for the indicated times. (c) The cells were stimulated with cerulein in the presence and absence of resistin for 1 h. NF-κB-DNA binding activity was determined by electrophoretic mobility shift assay (EMSA).

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