EZH2 Mediates miR-146a-5p/HIF-1α to Alleviate Inflammation and Glycolysis after Acute Spinal Cord Injury

Figure 5

EZH2 mediates miR-146a-5p/HIF-1α to alleviate inflammation and glycolysis in ASCI rats. (a) qRT-PCR detected EZH2, miR-146a-5p, and HIF-1α expressions in the Sham group and ASCI group. (b) Western blot detected the expression of H3K27me3 in the Sham group and ASCI group. (c) qRT-PCR was performed to detect miR-146a-5p expression. (d) Western blot detected the expression of HIF-1α. (e–g) ELISA was performed to detect inflammatory factor IL-6, TNF-α, and IL-17 levels in the cerebrospinal fluid of rats. (h) Metabolomics detected the relative levels of glycolytic metabolites G6P, F6P, SUCCINATE, and MALATE. vs. Sham group; ^# vs. ASCI group.

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