miR-708 Negatively Regulates TNFα/IL-1β Signaling by Suppressing NF-κB and Arachidonic Acid Pathways

Figure 1

TNFα/IL-1β modulates COX-2, mPGES-1, and miR-708 expression temporally in nondiseased lung cells. (a) Representative western blot of COX-2 and mPGES-1 protein expression from 0 to 48 hours after TNFα/IL-1β exposure in Beas2b cells. GAPDH served as a loading control. (b) RT-qPCR of mature miR-708 (blue) and ODZ4 (red) mRNA expression in Beas2b cells 0–48 hours post-TNFα/IL-1β exposure. miR-708 expression was normalized to U6 snRNA while ODZ4 mRNA was normalized to GAPDH mRNA and analyzed using the 2^-ΔΔCT method. miR-708 and ODZ4 levels were compared to 0 h treatments when determining significance; and , .

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