miR-708 Negatively Regulates TNF<i>α</i>/IL-1<i>β</i> Signaling by Suppressing NF-<i>κ</i>B and Arachidonic Acid Pathways

<div>miR-708 and AA signaling regulation is uncoupled in TNF<i>α</i>/IL-1<i>β</i>-modulated diseased lung cells. (a) Representative western blot of COX-2 and mPGES-1 protein expression from 0 to 48 hours after TNF<i>α</i>/IL-1<i>β</i> exposure in A549 cells. GAPDH served as a loading control. (b) RT-qPCR of mature miR-708 in Beas2b (blue, nondiseased) and A549 (red, diseased) cells 0–48 hours post-TNF<i>α</i>/IL-1<i>β</i> exposure. miR-708 expression was normalized to U6 snRNA and analyzed using the 2<sup>-<i>ΔΔ</i>CT</sup> method. <svg height="13.532pt" id="M26" style="vertical-align:-3.4295pt" version="1.1" viewbox="-0.0498162 -10.1025 68.0304 13.532" width="68.0304pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M486 158C486 177 478 202 466 220C413 228 386 236 336 262C386 288 413 297 466 304C478 323 486 347 485 366C470 376 444 381 422 380C389 338 368 319 321 288C323 345 329 372 349 422C339 442 322 461 305 470C289 461 271 442 262 422C281 372 287 345 290 288C243 319 222 338 189 380C167 381 142 376 125 366C125 347 133 322 145 304C198 296 225 288 275 262C225 236 198 227 145 220C133 201 125 177 126 158C141 148 167 143 189 144C222 186 243 205 290 236C288 179 282 152 262 102C272 82 289 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Mediators of Inflammation

fig2

Figure 2

Figure 2: miR-708 Negatively Regulates TNF<i>α</i>/IL-1<i>β</i> Signaling by Suppressing NF-<i>κ</i>B and Arachidonic Acid Pathways 

Mediators of Inflammation

miR-708 Negatively Regulates TNFα/IL-1β Signaling by Suppressing NF-κB and Arachidonic Acid Pathways

Figure 2