miR-708 Negatively Regulates TNFα/IL-1β Signaling by Suppressing NF-κB and Arachidonic Acid Pathways

Figure 3

miR-708 suppresses TNFα/IL-1β-induced arachidonic acid signaling partially through a positive feedback loop in diseased lung cells. (a) Representative western blot examining COX-2 and mPGES-1 protein expression in A549 cells transiently transfected with mock (left) or 25 nM miR-708 (right) in combination with TNFα/IL-1β for 0–48 hours. (b) ELISA measuring exogenous PGE₂ levels in A549 cells transiently transfected with mock (blue) or 25 nM miR-708 (red) and treated with TNFα/IL-1β for 0–48 hours. (c) Representative western blot examining COX-2 and mPGES-1 protein expression in A549 cells cotreated with vehicle (left) or 10 μM CEL (right) and TNFα/IL-1β for 0–48 hours. (d) ELISA measuring exogenous PGE₂ levels in A549 cells cotreated with vehicle (blue) or 10 μM CEL (red) and TNFα/IL-1β for 0–48 hours. , , , and ; .

(a)

(b)

(c)

(d)