miR-708 Negatively Regulates TNFα/IL-1β Signaling by Suppressing NF-κB and Arachidonic Acid Pathways

Figure 5

miR-708 and NF-κB inhibition decreases IKKβ and COX-2 protein expression, respectively, in TNFα/IL-1β-treated diseased lung cells. (a) Representative western blot examining COX-2 and IKKβ (IKK-b) protein expression in A549 cells transiently transfected with mock (left) or 25 nM miR-708 (right) in combination with TNFα/IL-1β for 0–48 hours. (b) Representative western blot examining COX-2 and IKKβ (IKK-b) protein expression in A549 cells transiently transfected with mock (left), 25 nM miR-708 (middle), or 10 μM BAY-11-7082 in combination with TNFα/IL-1β for 0–24 hours. (c) Quantification of IKKβ protein expression from representative western blot (b) and others (data not shown). IKKβ expression was normalized to GAPDH protein expression and TNFα/IL-1β 0 h (left black bar). and , .

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