miR-708 Negatively Regulates TNFα/IL-1β Signaling by Suppressing NF-κB and Arachidonic Acid Pathways

Figure 6

miR-708 suppresses PGE₂ and IL-6 secretion by inhibiting NF-κB signaling in diseased lung cells. (a) Relative NF-κB promoter reporter NanoLuc luciferase activity in A549 cells. Relative luciferase activities were measured in response to mock (blue), 25 nM miR-708 (red), or 10 μM BAY-11-7082 (green) treatment for 0 or 5 h. Data were normalized to total protein and mock 0 h samples. Differences were compared between samples within 0 and 5 h time points. (b) ELISA measuring exogenous PGE₂ levels in A549 cells cotreated with vehicle (blue), 25 nM miR-708 (red), or 10 μM BAY-11-7082 (green) and TNFα/IL-1β for 0–48 hours. (c) ELISA measuring exogenous IL-6 levels in A549 cells cotreated with vehicle (blue), 25 nM miR-708 (red), or 10 μM BAY-11-7082 (green) and TNFα/IL-1β for 0–48 hours. and , .

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