Curcumin Antagonizes Glucose Fluctuation-Induced Renal Injury by Inhibiting Aerobic Glycolysis via the miR-489/LDHA Pathway

Figure 5

Cur prevented GF-triggered aerobic glycolysis via the miR-489/LDHA axis in HEK-293 cells. (a–c) RT-qPCR and WB results showed that Cur could alleviate GF-induced increase of LDHA in a dose-dependent manner at 48 h. (d) Viability of HEK-293 cells was tested by CCK8 at 48 h. (e) TNF-α and IL-1β at 48 h. (f) Levels of oxidative stress marker, including MDA and mitochondrial and cytoplasmic SOD at 48 h. (g) Apoptosis index was detected by flow cytometry at 48 h. Downregulation of miR-489 or upregulation of LDHA could weaken the protective effects of Cur in the fields of cell viability, inflammation injury, oxidative stress, and apoptosis. (h, i) The suppression of aerobic glycolysis by Cur treatment was prevented by the miR-489 inhibitor or LDHA pcDNA in HEK-293 cells. Each error bar reflects the SEM of at least three independent sets.

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